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李廣存

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姓 名: 李廣存
職 稱: 研究員
電 話: 010-82105955
郵 箱: liguangcun@caas.cn
研究方向: 馬鈴薯遺傳育種與種質(zhì)創(chuàng)制
最高學(xué)歷: 農(nóng)學(xué)博士
導(dǎo)師類別: 碩士、博士導(dǎo)師
研究領(lǐng)域: 蔬菜學(xué)

個人簡介

       1972年7月生,山東省菏澤人,博士、研究員、博士生導(dǎo)師、中國農(nóng)業(yè)科學(xué)院“創(chuàng)新型領(lǐng)軍人才”。1995年獲東北農(nóng)業(yè)大學(xué)學(xué)士學(xué)位,2002年獲山東大學(xué)碩士學(xué)位,2006年獲中國農(nóng)科院博士學(xué)位。1995年7月至2014年12月在山東省農(nóng)業(yè)科學(xué)院工作,2015年1月被人才引進至中國農(nóng)科院蔬菜花卉研究所。現(xiàn)任中國農(nóng)科院蔬菜花卉研究所馬鈴薯遺傳育種與栽培創(chuàng)新團隊首席科學(xué)家和課題組長、農(nóng)業(yè)農(nóng)村部薯類作物生物學(xué)和遺傳育種重點實驗室副主任;兼任國家馬鈴薯產(chǎn)業(yè)科技創(chuàng)新聯(lián)盟秘書長,中國作物學(xué)會馬鈴薯專業(yè)委員會副秘書長兼生物技術(shù)學(xué)部部長、中國農(nóng)科院園藝學(xué)科學(xué)位評定委員會委員等。

       主要從事馬鈴薯種質(zhì)資源的篩選評價、新種質(zhì)創(chuàng)制和分子育種工作,并在馬鈴薯新種質(zhì)創(chuàng)制、基因組學(xué)、重要性狀遺傳解析與基因挖掘、馬鈴薯與病原分子互作等方面取得了重要成果。先后獲得省部級以上科技獎勵6項,其中國家科技進步二等獎1項。在Nature、Nature Genetics、Molecular plant、Plant Cell、Plant Biotechnology Journal、中國農(nóng)業(yè)科學(xué)等期刊上發(fā)表論文100余篇(其中SCI收錄40余篇)。


科研情況

      針對馬鈴薯關(guān)鍵共性技術(shù)瓶頸和產(chǎn)業(yè)問題,以為馬鈴薯產(chǎn)業(yè)綠色健康發(fā)展提供理論支撐和關(guān)鍵技術(shù)為目標(biāo),從馬鈴薯種質(zhì)資源收集評價到新品種培育的多個環(huán)節(jié)開展研究。評價篩選和創(chuàng)制了馬鈴薯抗病抗逆優(yōu)質(zhì)等特異新種質(zhì)100余份;創(chuàng)建了高通量BAC-end測序輔助基因組組裝和物理圖譜構(gòu)建新方法,構(gòu)建了茄科作物第一張基因組完成圖(Potato-T2T/DM8.1),聯(lián)合開發(fā)了異交植物基因高效定位新算法工具OcBSA,主要骨干參與完成了四倍體普通栽培種馬鈴薯的基因組圖譜繪制等,為馬鈴薯的遺傳育種研究奠定了基因組學(xué)基礎(chǔ);定位克隆了R3b、StGolS1、StNADC、Ro等抗晚疫病、耐寒、熟性、薯形等重要性狀的功能基因(位點),初步解析了馬鈴薯抗晚疫病、抗旱、耐寒、優(yōu)質(zhì)及表觀等性狀的遺傳基礎(chǔ),突破了同源四倍體難以進行標(biāo)記開發(fā)和重要性狀遺傳解析的難題,建立了抗病、抗逆、熟性等標(biāo)記輔助育種體系并用于馬鈴薯新品種培育。集成創(chuàng)新了水肥高效利用的栽培管理技術(shù),主要完成人育成了中薯27、中薯49、中薯早35、中薯早39等多個馬鈴薯新品種。已培養(yǎng)研究生和博士后24人,已畢業(yè)12人,在讀在站研究生博士后14人。

主要成果

  1. Zhang LK?; Duan YF?; Zhang ZW; Zhang L; Chen SM; Cai CC; Duan SG; Zhang K; Li GC*; Cheng F*. OcBSA: an NGS-based Bulk Segregant Analysis Tool for Outcross Populations. Molecular Plant. 2024, https://doi.org/10.1016/j.molp.2024.02.011.

  2. Hu J, Duan YF, Hu JX, Zhang SQ, Li GC* Identification and expression analysis of the sucrose synthase and sucrose phosphate synthase gene family in potato. Metabolites. 2024, 14, 70. https://doi.org/10.3390/metabo14010070

  3. Yan WY?, Qin JH?, Jian YQ, Liu JG, Bian CS, Jin LP, Li GC*. Analysis of Potato Physiological and Molecular Adaptation in Response to Different Water and Nitrogen Combined Regimes. Plants. Plants 2023, 12, 1671. https://doi.org/10.3390/plants12081671

  4. Yang XH?, Zhang LK?, Guo X, Xu JF, Zhang K, Yang YQ, Yang Y, Jian YQ, Dong DF, Huang SW, Cheng F*, and Li GC*. The gap-free potato genome assembly reveals large tandem gene clusters of agronomical importance in highly repeated genomic regions. Molecular Plant, 2023, 16, 314–317. https://doi.org/10.1016/j.molp.2022.12.010

  5. Bao ZG?, Li CH?, Li GC?, Wang P, Peng Z, Cheng L, Li HB, Zhang ZY, Li YY, Huang W, Ye MW, Dong DF, Cheng ZK, Peter VanderZaag, Jacobsen Evert, Christian W. B. Bachem, Dong SM, Zhang CZ, Huang SW*, and Zhou Q*. Genome architecture and tetrasomic inheritance of autotetraploid potato. Molecular Plant, 2022, 15, 1211–1226.

  6. Yang XH?, Yang Y?, Ling J, Guan JT, Guo X, Dong DF, Jin LP, Huang SW, Liu J * and Li GC*. A high-throughput BAC-end analysis protocol (BAC-anchor) for profiling genome assembly and physical mapping. Plant Biotechnology Journal, 2020 (18): 364–372.

  7. He FY, Xu JF, Jian YQ, Duan SG, Hu J, Jin LP*, and Li GC*. Overexpression of galactinol synthase 1 from Solanum commersonii (ScGolS1) confers freezing tolerance in transgenic potato, Horticultural Plant Journal, 2022, doi: doi.org/10.1016/j.hpj.2022.05.005

  8. He FY?, Duan SG?, Jian YQ, Xu JF, Hu J, and Li GC*. Genome-wide identification, structural and gene expression analysis of the 14-3-3 gene family in Potato (Solanum tuberosum L.). BMC Genomics, 2022. 23:811 https://doi.org/10.1186/s12864-022-09037-y

  9. Fan GY?, Wang QR?, Xu JF, Chen N, Zhu WW, Duan SG, Walter S De Jong, Jin LP*, and Li GC*. Fine mapping and candidate gene prediction of tuber shape controlling Ro locus based on integrating genetic and transcriptomic analyses in potato. International Journal of Molecular Science, 2022, 23, 1470. https://doi.org/10.3390/ijms23031470

  10. Yan WY?, Jian YQ?, Duan SG, Guo X, Hu J, Yang XH*, and Li GC*. Dissection of plant hormone signal transduction network in late blight resistance potato genotype SD20 and prediction of key resistance genes. Phytopathology, 2022. https://doi.org/10.1094/PHYTO-04-22-0124-R

  11. Yang Y?, Yang XH?, Guo X, Hu XX, Dong DF, Li GC*, Xiong XY*. Exogenously applied methyl jasmonate induces early defense related genes in response to Phytophthora infestans infection in potato plants, Horticultural Plant Journal, 2022, doi: https://doi.org/10.1016/j.hpj.2022.04.003

  12. Hui ZM, Xu JF, Jian YQ, Bian CS, Duan SG, Hu J, Li GC* and Jin LP*. Identification of long-distance transport signal molecules associated with the plant maturity of tetraploid cultivated potato (Solanum tuberosum L.). Plants. 2022. 11, 1707. https://doi.org/10.3390/plants11131707

  13. Qin JH, Bian CS, Duan SG, Wang WX, Li GC*, and Jin LP*. Effects of different rotation cropping systems on potato yield, rhizosphere microbial community and soil biochemical properties. Frontiers in Plant Science,2022, 13:999730. https://doi.org/10.3389/fpls.2022.999730

  14. Jian YQ, Yan WY, Xu JF, Duan SG, Li GC*, and Jin LP*. Genome-wide simple sequence repeat markers in potato: abundance, distribution, composition, and polymorphism. DNA Research, 2021, 28(6) dsab020, https://doi.org/10.1093/dnares/dsab020

  15. Duan YF, Duan SG, Xu JF, Zheng JY, Hu J, Li BJ, Li GC*, and Jin LP*. Late blight resistance evaluation and genome-wide assessment of genetic diversity using SSR and SNP markers in wild and cultivated potato species. Frontiers in Plant Science, 2021. 12: 710468. doi: 10.3389/fpls.2021.710468

  16. Zheng JY, Duan SG, Armstrong MR, Duan YF, Xu JF, Chen XW, Hein I, Jin LP* and Li GC*. New findings on the resistance mechanism of an elite diploid wild potato species JAM1-4 in response to a super race strain of Phytophthora infestans. Phytopathology, 2020,110. doi: 10.1094/PHYTO-09-19-0331-R.

  17. Zheng JY, Yang Y, Guo X, Jin LP, Xiong XY, Yang XH*, and Li GC*. Exogenous SA initiated defense response and multi-signaling pathway in tetroploid potato SD20. Horticultural Plant Journal, 2020. 6 (2): 111–122.

  18. Yang XH?, Chen L?, Yang Y, Guo X, Chen GX, Xiong XY, Dong DF*, and Li GC*. Transcriptome analysis reveals that exogenous ethylene activates immune and defense responses in a high late blight resistant potato genotype. Scientific Reports, 2020, 10:21294. doi.org/10.1038/s41598-020-78027-5.

  19. Yang XH, Guo X, Chen GX, Dong DF, Liu F, Yang YJ, Yang Y* and Li GC*. Comparison of defense responses of transgenic potato lines expressing three different Rpi genes to specific Phytophthora infestans races based on transcriptome profiling. PeerJ, 2020. 8:e9096 http://doi.org/10.7717/peerj.9096

  20. Hou Q, Wang WX, Yang Y, Hu J, Bian CS, Jin LP, Li GC*, and Xiong XY*. Correlation analysis of rhizosphere microbial diversity and soil physical-chemical properties provides insight into fertilization management during potato cultivation”. European Journal of Soil Biology, 2020, (98) 103176. doi.org/10.1016/j.ejsobi.2020.103176

  21. Duan YF, Duan SG, Xu JF, Zheng JY, Hu J, Li GC*, and Jin LP*. Comparative transcriptome profiling reveals compatible and incompatible patterns of potato toward Phytophthora infestans. G3/Genes Genomes Genetics, 2020 (10): 623-634.

  22. “早熟優(yōu)質(zhì)多抗馬鈴薯新品種選育與應(yīng)用”獲2017年度國家科技進步二等獎(排名第5)

  23. “抗病耐凍早熟馬鈴薯育種技術(shù)的建立及新品種選育” 獲2015年度中華農(nóng)業(yè)科技一等獎(排名第7)

科研項目(近五年)

  1. 國家重點研發(fā)課題(主持):馬鈴薯優(yōu)質(zhì)多抗新種質(zhì)創(chuàng)制與應(yīng)用(2023YFD1201505),297萬元,2023.10-2027.12

  2. 國家馬鈴薯產(chǎn)業(yè)技術(shù)體系崗位科學(xué)家(CARS-9-P11,主持),315萬元,2021.1-2025.12

  3. 國家自然科學(xué)基金面上項目(主持):StNADC1基因及其互作蛋白共調(diào)控馬鈴薯植株生理成熟的分子機制研究(32072119),69.6萬元,2021.1-2024.12

  4. 國家自然科學(xué)基金國際合作重點項目(主持):馬鈴薯重要農(nóng)藝性狀遺傳區(qū)段解析與優(yōu)異種質(zhì)發(fā)掘(31561143006),273.2萬元,2016.1-2020.12

  5. 中國農(nóng)業(yè)科學(xué)院“創(chuàng)新型領(lǐng)軍人才”項目(主持),400萬元,2024.1-2028.12

  6. 中國農(nóng)業(yè)科學(xué)院創(chuàng)新工程項目(主持):馬鈴薯遺傳育種與栽培創(chuàng)新團隊(CAAS-ASTIP-IVFCAAS),650萬元,2022.1-2026.12

 

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      博學(xué)之,審問之,慎思之,明辨之,篤行之。--《禮記·中庸》

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